1.1 Overview of marine biotechnology: applications, sectors, and the “blue biotech” value chain.
1.2 Marine ecosystems and biodiversity hotspots: implications for bioprospecting.
1.3 Main biological groups of interest: bacteria, archaea, microalgae, macroalgae, invertebrates.
1.4 Adaptations to the marine environment: salinity, pressure, temperature, UV radiation, and its value Biotechnology.
1.5 Blue bioeconomy concepts: sustainability, traceability, and scalability.
1.6 Data quality and reproducibility: sampling, metadata, and bias control.
1.7 Introduction to the chemistry of marine natural products: classes and functions.
1.8 Valorization routes: from Biological resource to ingredient, biomaterial, or drug.
1.9 Risks and ethics: environmental impact, access to resources, and equity.
1.10 Designing a project pipeline: objective → resource → method → ​​validation → product.

2.1 Sampling strategies by habitat: coastal, pelagic, deep-sea, estuaries.
2.2 Campaign design: logistics, contamination control, and sample preservation.
2.3 Conservation protocols: cryopreservation, DNA/RNA stabilization, fixation.
2.4 Marine biobanks: traceability, coding, QA/QC, and chain of Custody.
2.5 Isolation and culture: techniques for difficult microorganisms and microalgae.
2.6 Strain collections: authentication, purity, and maintenance criteria.
2.7 Environmental metadata: oceanographic variables and their integration with results.
2.8 FAIR data management: repositories, Standards and governance.
2.9 Sampling impact assessment: minimization and good practices.
2.10 Applied biobank plan: operational design and documentation.

3.1 Marine microbial ecology: communities, niches, and functions.
3.2 Extremophilic microorganisms: value for enzymes and metabolites.
3.3 Traditional culture vs. “culturomics”: expansion of cultivable diversity.
3.4 Production of secondary metabolites: triggers, stress, and co-culture. data-start=”2409″ data-end=”2412″ />3.5 Marine enzymes: lipases, proteases, cellulases, chitinases; Stability in salt/temperature.
3.6 Biofilms and adhesives: biological bases and applications.
3.7 Marine bioremediation: hydrocarbons, metals, nutrients, and microplastics.
3.8 Strain engineering and optimization: non-advanced strategies and productivity control.
<strong data-start="2768" 3.9 Microbial biosensors: environmental detection and process control.
3.10 Applied case: strain selection and industrial valorization route.

4.1 Metagenomics: design, assembly, annotation, and biases.
4.2 Metatranscriptomics and proteomics: when they add value and how to interpret them.
4.3 Marine metabolomics: chemical profiles and biomarkers.
4.4 Strain genomics: identification, biosynthetic clusters, and bioproductive potential. 4.5 Diversity Analysis: Alpha/Beta Diversity, Networks, and Functional Ecology.
4.6 Data-Driven Discovery: Prioritizing Candidates by “Omics”.
4.7 Multi-Omics Integration: Hypothesis, Causality, and Experimental Validation.
4.8 Reproducible Pipelines: Version Control, Notebooks and documentation.
4.9 Interpretation for R&D: from bioinformatics results to laboratory decisions.
4.10 Bioinformatics laboratory: complete workflow and technical report.

5.1 Bioprospecting strategies: guided by taxonomy, ecology, omics, or phenotype.
5.2 Extract preparation: polarity, fractionation, and degradation control.
5.3 Biological assays: antimicrobial, anti-inflammatory, cytotoxic, antioxidant, and enzymatic.
5.4 Dereplication: Avoid rediscoveries and accelerate selection.
5.5 Isolation and purification: chromatography, purity criteria, and yields.
5.6 Structural elucidation: fundamentals and interpretation of results (applied view).
5.7 Hit-to-lead optimization: power, selectivity, and stability.
<strong data-start="4491" 5.8 Safety assessment: toxicity, allergenicity, and preliminary profiles.
5.9 Scale-up of production: cultivation, semichemical synthesis, or sustainable alternatives.
5.10 Candidate dossier: evidence, traceability, and development plan.

6.1 Bioprocess design: upstream vs downstream and productivity KPIs.
6.2 Fermentation under marine conditions: salinity, aeration, control, and contamination.
6.3 Microalgae cultivation: photobioreactors, raceways, harvesting, and stability.
6.4 Critical parameters: pH, DO, nutrients, light, temperature, Foam.
6.5 Process intensification: fed-batch, perfusion, co-culture (applied view).
6.6 Downstream: extraction, clarification, filtration, drying, and formulation.
6.7 Process economics: balances, yields, energy, and cost per kg.
<strong data-start="5418" 6.8 Quality control: specifications, impurities, traceability, and documentation.
6.9 Scaling up: from laboratory to pilot and industrial; transfer risks.
6.10 Plant/operation design: conceptual layout and validation plan.

7.1 Macroalgae: biology, cultivation, and supply chains.
7.2 Microalgae: strain selection, productivity, and robustness.
7.3 Biomolecules of interest: pigments, lipids, proteins, polysaccharides, functional compounds.
7.4 Applications: nutraceuticals, cosmetics, food, fertilizers, and materials. 7.5 Biomass processing: stabilization, green extraction, and fractionation.
7.6 Quality and safety: contaminants, metals, and standardization criteria.
7.7 Integrated farming (IMTA): synergies with aquaculture and sustainability.
7.8 Certification and claims: Typical requirements for ingredients and products.
7.9 Product design: formulation, stability, and shelf life.
7.10 Applied case: seaweed ingredient with a technical-commercial plan.

8.1 Foundations of modern aquaculture: species, systems, and risks.
8.2 Functional nutrition: marine ingredients, digestibility, and performance.
8.3 Probiotics and the microbiome: monitoring health and performance.
8.4 Diagnosis and surveillance: detection methods and health control.
<strong 8.5 Vaccines and biotechnological tools: applied vision and limitations.
8.6 Applied genetics: selection, traceability, and productivity improvement (conceptual approach).
8.7 Animal welfare and biosecurity: procedures and auditing.
8.8 Antimicrobial resistance: prevention and responsible management.
8.9 Environmental impact: effluents, leaks and mitigation.
8.10 Intervention plan: health-productivity improvement with metrics.